The Mastodons compete in 16 sports. Purdue University Fort Wayne is an NCAA Division I institution competing in the Horizon League and Midwestern Intercollegiate Volleyball Association. Sports offered consist of baseball, basketball, cross country, golf, soccer, softball, track and field (indoor and outdoor,) and volleyball.
The mission of Purdue Fort Wayne’s athletics is to foster an environment of growth in the areas of academics, athletics, and social responsibility. Mastodon coaches, staff, and student-athletes work to enhance Purdue Fort Wayne's reputation by reaching for academic and athletic excellence. The Mastodon Academic Performance Center provides ongoing academic advising, as well as support for career and personal development. The ultimate goal is to support the accomplishment of a higher-education degree.